spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Wrch-1 regulates JNK activation. (A) Wrch-1 depletion inhibits JNK activation. Wounding-induced JNK activation was determined as described in Materials and Methods. Blot is representative of three independent experiments. Levels of phosphorylated Jun from two independent experiments were quantified and normalized to tubulin levels. *P<0.05, two-tailed t test. (B) JNK activity is necessary for HelaS3 migration. JNK activity was inhibited by 2 µM of SP600125 (with overnight pre-treatment). Other conditions as in Fig. 5D. *P<5x10–7, two-tailed t-test. Data are representative of four independent experiments. (C) Inhibition of JNK activity does not affect focal adhesion formation in HeLaS3 cells. Cells were treated with either DMSO or SP600125 (20 µM) for 24 hours. The number of focal adhesions per cell was determined as in Fig. 2D. Data are representative of two independent experiments.





Right arrow Return to article