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Figure 1


Fig. 1. Subcellular localization of BAF in various human cell lines. (A) Western blotting using unpurified (`before', left panel) and affinity-purified (`after', middle and right panels) anti-BAF-3273-6 antibody. Whole extract (total), the cytoplasmic fraction (cytosol), and the nuclear fraction (nucleus) from HeLa cells (left and middle panels) and GFP-BAF-expressing HeLa cells (right panel) were analyzed. (B) Indirect immunofluorescence staining of HeLa cells using affinity-purified anti-BAF antibody (upper panels) and the same antibody pretreated with epitope peptides (lower panels). Left panels are anti-BAF staining (green in merged images), middle panels are DAPI staining for DNA (blue in merged images), and right panels are merged images. (C) Indirect immunofluorescence staining of various human cell types using affinity-purified anti-BAF antibody. Arrows represent cells with prominent nuclear signals and arrowheads represent cells with prominent cytoplasmic signals. Asterisks indicate cells with uniform signals. Bar, 40 µm.





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