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Figure 7


Fig. 7. Western blotting analysis for cell-cycle-related proteins. HeLa cells were treated with luciferase RNAi as control (left lane) or BAF RNAi (right lane) using Oligofectamine. 25 µg cell extract (corresponding to 5x104 cells) was analyzed by western blotting using the antibodies indicated on the left. Amounts of corresponding protein in BAF RNAi cells are represented on the right as percentages of the control. Cell extracts were prepared in the homogenizing buffer without (A) or with (B) phosphatase inhibitors.





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