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Fig. 3. GFP localization identifies candidate genes involved in nuclear differentiation. (A) A developmental cDNA library, inserted into GFP expression vector pCGF1, was transformed into Tetrahymena to generate panels of transformants that were selected in 96-well tissue culture plates. Replicates of these panels were crossed to wild-type strain CU428 and visualized for GFP-nuclear localization using an inverted fluorescence microscope. (B) Representative images of mating cells expressing four different GFP fusions to coding sequences identified as histone H2B, histone H4, micronuclear linker histone (MLH), PDD2 and one novel gene, LIA3, are shown. For each pair or exconjugant, a series of DIC, green fluorescence and DAPI-stained images is presented. White arrowheads indicate the different nuclei for positional reference between images.
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