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Fig. 4. LIA genes encode novel proteins expressed during nuclear differentiation. (A) Each lane contains 20 µg total RNA isolated from vegetatively growing (gr), starved (st) or conjugating cells at the indicated time after mixing of complementary mating types. The estimated transcript sizes are given to the left of each scanned autoradiogram. (B) The gene structures of LIA1 to LIA5 are depicted: open boxes, coding regions; gaps spanned with carets, introns; solid black bars, transcribed non-translated regions. The size is given above each intron. The divisions of the scale bar represent 250 bp. All non-translated sequences, introns and the LIA4 polyadenylation site (pA) were verified by our cloned cDNAs or database cDNA sequence. Gray bars underline the sequences found in the original cDNAs recovered in the screen, which were radiolabeled as probes for the northern blot analysis shown in A.
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