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Figure 5


Fig. 5. Lia proteins exhibit dynamic localization during conjugation. N-terminal GFP fusion proteins were expressed from the pIGF-LIA expression cassette shown at the top. Representative DIC, GFP and DAPI-stained image series are shown for LIA1, LIA3, LIA4 and LIA5 as indicated. (A) Early macronuclear development 7-8 hours into conjugation. (B) Late macronuclear development 12-14 hours into conjugation. White arrows indicate conjusome (C) localization. White arrowheads denote representative punctate foci that are similar to Pdd1p-containing foci.





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