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Figure 6


Fig. 6. Lia proteins co-localize with Pdd1 in DNA rearrangement foci. Conjugating cells expressing the indicated GFP-Lia fusion proteins were fixed with paraformaldehyde to preserve GFP fluorescence and incubated with Pdd1p-specific antibodies, detected with Rhodamine-conjugated anti-rabbit secondary antibodies. Images of GFP or immunofluorescence are shown separately and merged with the corresponding DAPI-stained images and pseudocolored (GFP-Lia, green; Pdd1p, red; DAPI, blue) to highlight overlapping localization (yellow). White arrows are given for positional reference between corresponding images.





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