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Fig. 1. Confocal images showing small areas of Drosophila syncytial embryos that were taken from GFP::Cdc27 (A) or GFP::Cdc16 (B) transgenic flies with or without treatment with a 2.5 mM final intracellular concentration of colchicine. Drosophila GFP::Cdc27 associates with mitotic chromosomes (A, panels 1 and 7, 2 and 8, arrows), but GFP::Cdc16 does not (B, panels 1 and 7, 2 and 8, arrows indicate shadow regions), both GFP::Cdc27 and GFP::Cdc16 associate with nuclear envelope membrane (open arrowheads in A,B panels 1 and 7). Colchicine treatment clearly increases the association of GFP::Cdc27 with mitotic chromosomes (A, panels 3 and 9 arrows) compared with the metaphase control (A, panels 1 and 7, arrows). The localisation of the Drosophila GFP::Cdc16 is not affected by the same treatment (compare B, panels 3 and 9 with 1 and 7). A,B panels 1-3:merge images (GFP::Cdc27 or GFP::Cdc16 in green, chromosomes in red), A,B panels 4-6: Rhodamine-H1-labelled chromosomes in white; A,B panels 7-9: GFP::Cdc27 or GFP::Cdc16 in white; A,B, panels 7 and 8: non-colchicine treated embryos; A,B, panel 9: colchicine-treated embryos. The developmental stage of syncytial embryos for each experiment was about nuclear division cycle 8 or 9. Bars, 10 µm.

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