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Figure 4


Fig. 4. Intracellular distribution of Chs3p in the chs4{Delta} mutant. (A) Localization of Chs3p-GFP in the indicated strains. Arrowheads indicate Chs3p-GFP localization at the neck in the chs4{Delta} strain. Note the reduced intensity of the label compared with the wild type. (B) immunofluorescence localization of Chs3p-3xHA in wild-type, chs4{Delta} and chs5{Delta} strains (Y1306 background). Note the high definition of staining in the wild type in contrast to the diffuse and dotted pattern in the chs4{Delta}. Chs3p was retained prominently in Golgi vesicles in the chs5{Delta}. Data from wild type and chs4{Delta} are also expressed in graph format as percentages (n=230/240) of cells with the indicated distribution pattern, referred to the number of cells with defined staining. Discrete distribution refers to cells presenting staining sharply defined at the plasma membrane; Diffuse, polarized staining not sharply defined; Bud, cells showing diffuse staining at bud periphery.





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