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Fig. 7. Effect of YPT32 overexpression on chitin synthesis. (A) Calcofluor staining of the indicated strains. Upper row shows the strains transformed with the pRS314 plasmid and the lower row those transformed with pRS314-GAL1-YPT32. Cells were grown in galactose and stained as indicated. (B) Chs3p-GFP localization after YPT32 overexpression. All the indicated strains contained the YpLac111-CHS3-GFP plasmid and the additional indicated plasmids. Note that such overexpression does not modified Chs3p-GFP accumulation in the neck in either wt or chs4
strains. Cells were grown in galactose and observed microscopically as described.