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Figure 9


Fig. 9. Chs4p distribution in the absence of endocytosis. Calcofluor staining and Chs4p distribution in the end4{Delta} chs4{Delta} mutant transformed with pRS315 plasmids containing either the CHS4-GFP or CHS4{Delta}590-GFP constructs. Chs4p-GFP distribution did not change as a result of the end4{Delta} mutation, independently of the C-terminal region of the protein (compare with Fig. 3A). Yeast cells were grown at 25°C.





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