spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Shiga toxin B-subunit is transported via SNX1-positive endosomes to the TGN. Fluorescence-labeled Shiga toxin B-subunit (FITC-STxB, green channel) was surface-bound to HeLa cells at 4°C and subsequently allowed to internalize for the indicated times at 37°C or 19.5°C; cells were fixed and immunolabeled for endogenous SNX1 (Alexa594, red channel) and imaged using confocal microscopy. Maximum projections of eight to ten optical z-slices (480 nm z-separation) are shown. (A) Cells were fixed without incubation at 37°C (0 minutes). (B) After 10 minutes at 37°C, the Shiga toxin B-subunit extensively colocalized with the SNX1-positive endosomal structures (yellow color in merged images). Note the subset of FITC-STxB tubules negative for SNX1 (arrowheads in B and C). (C) Shiga toxin B-subunit accumulated in SNX1-positive endosomes when incubated at 19.5°C for 1 hour. When incubated for longer times at 37°C (D,E), it displayed a TGN-like enrichment at the juxtanuclear area. (F) At these time points, Shiga toxin B-subunit colocalised extensively with TGN46 (Alexa594, red). Bars, 10 µm.





Right arrow Return to article