spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Quantification of the defect in endosome-to-TGN transport in SNX1-suppressed cells. Toxin-uptake assays using control and SNX1-suppressed HeLa cells were performed as described. After fixation and immunolabeling with anti-SNX1 (Alexa594, red channel), samples were inspected by epifluorescence microscopy and scored blindly for either a TGN-like enrichment (x) or a vesicular appearance (*). Cells marked `o' were not scored because of low toxin levels. (A,B) Scoring phenotypes; FITC-STxB in green channel and TGN46 (Alexa594) in red channel for control and SNX1-suppressed cells after 30 minutes of uptake. (C) Representative data for a set of control and SNX1-suppressed cells (n>600) after 30 minutes of toxin uptake. (D) TGN-like accumulation of FITC-STxB over time (n>600 for each sample; scored for 30, 60 and 120 minutes) for SNX1-suppressed and control cells. (E) Colocalization of FITC-STxB and TGN46, digitally quantified from confocal images using MetaMorph software (see Materials and Methods for details). In control cells, 37±0.4% of FITC-STxB colocalized with TGN46 after 30 minutes of internalization, whereas SNX1-suppressed cells showed 12±1% colocalization. Values given are ± the standard deviation and were determined from the mean of individual visual fields for a total of n>50 cells. Bar, 20 µm.





Right arrow Return to article