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Figure 2


Fig. 2. Retromer protein Vps26 on STxB-containing endosomes. (A) Cy3-STxB was bound to HeLa cells kept on ice that were then transferred to 37°C for 10 minutes, fixed and labeled for the indicated proteins. Bar, 10 µm. (B) Magnification of boxed areas in A. Insets show detailed view of an STxB-containing tubular structure. (B') Pair-wise overlays of images shown in B. Note that TfR overlaps with vacuolar parts of STxB structures (arrows), whereas Vps26 overlaps with tubular parts (arrowheads). (B,B') Bar, 5 µm.





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