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Figure 5


Fig. 5. Immunofluorescence analysis of STxB transport in Vps26 siRNA-treated cells. (A,B) Cy3-labeled STxB (red) was incubated for 45 minutes at 37°C with (A) mock-transfected or (B) Vps26 siRNA-transfected cells. After fixation, cells were stained for Vps26 (green) and CTR 433 (blue), a marker of the Golgi apparatus. In control cells, STxB accumulated in Golgi membranes, whereas it failed to do so in Vps26 siRNA-treated cells. There, it accumulated in peripheral structures. Bars, 10 µm.





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