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Fig. 7. Ultrastructural localization of STxB in Vps26 and clathrin RNAi conditions. (A-E) STxB was incubated for 45 minutes at 37°C with (A) mock transfected cells, (B,C) Vps26-depleted and (D,E) clathrin-depleted cells. After fixation and cryosectionning, preparations were labeled with anti-STxB antibody (A-E, 15-nm gold particles) and with anti-TfR antibody (C-E, 10-nm gold particles). In C, arrows indicate clusters of TfR-specific labeling, arrowheads indicate clusters of STxB-specific labeling. In D, the star indicates a tubule in which both TfR and STxB can be detected, arrowhead indicates the vacuolar endosome from which the tubule emanates. Note that in Vps26-depleted cells, STxB is found throughout the cytoplasm, whereas it is often localized close to multivesicular endosomes in clathrin-depleted cells. Bars, 200 nm.
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