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Figure 1


Fig. 1. Extracellular secretion of epimorphin in mammary stromal cells. (A) Epimorphin is produced in the E-cadherin-negative compartment in the mammary gland. Cryosections (left panel) and primary cells (right panel) of glands from mid-pregnant (day 13) mice (ICR, Shimizu Laboratory Supplies) were stained with goat anti-E-cadherin antibodies (E-cadherin; green) and affinity-purified rat anti-epimorphin antibodies (Epm; red). Non-immunized rat serum and secondary antibodies did not label cells. Bars, 100 µm. (B) Secretion of the 30 kDa soluble epimorphin but not of highly similar syntaxin 1A. Epimorphin-producing mammary cell populations were transfected with expression plasmid for T7-tagged epimorphin or syntaxin 1A (TE and TS, respectively), and proteins were immunoprecipitated from the whole-cell culture (cell + sup), cell (cell) or supernatant (sup) with pan-syntaxin antibodies followed by western blot analysis using anti-T7 mAb. Loaded amount of supernatant was five times higher than that of cell lysate.





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