spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Identification of critical amino acids for epimorphin secretion. (A) Comparison of the sequences close to the cleavage site of epimorphin (Epm) and conserved syntaxin family members. Syntaxin 1A, syn1; syntaxin 3 (NM001025307), Syn3; and syntaxin 4 (NP033320), Syn4. Histidine H246 (red) is conserved in epimorphin from mouse (m, D10475), human (h, D14582), quail (q, AB076670) and sheep (s, E32546); other syntaxins have arginine R246 (blue). Adjacent to H246, the KEE sequence (red-yellow-red) is also conserved in epimorphin, whereas the VXD sequence (blue-black-blue) occupies this position in syntaxin 1A and syntaxin 3. (B) H246 is crucial for processing and secretion of epimorphin. The H246R mutation (CAT->AGG) in the T7-epimorphin sequence dramatically suppressed the secretion of 30 kDa epimorphin. The R246H (AGG->CAT) mutation in T7-syntaxin-1A resulted in the secretion of a 30 kDa form.





Right arrow Return to article