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Figure 1


Fig. 1. Targeting of the Hrk locus. (A) Schematic diagram (to scale) of the Hrk-targeting construct, the wild-type (WT) Hrk locus and the targeted locus. Dashed lines demarcate regions of the WT locus used in the targeting construct. Location of 5' and 3' external probes for genomic Southern blot analysis and expected fragment sizes from indicated restriction digests are shown. B, BglII; Sc, ScaI; N, NcoI. (B) Confirmation by Southern blotting using 5' genomic probes of the loss of both wild-type Hrk alleles in Hrk–/– mice. (C) Northern blot analysis of polyA+ mRNA extracted from brains of neonatal wild-type (+/+), Hrk+/– (+/–) and Hrk–/– (–/–) littermates. Gapdh RNA was loaded as control.





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