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Figure 2


Fig. 2. HRK is not required for cytokine withdrawal-induced death of haematopoietic progenitors. (A) Foetal liver cells from E13.5 wt, Hrk+/– and Hrk–/– embryos were plated in soft agar and colonies scored 7 days after the addition of cytokines. Cytokines were added to the cultures at the time of plating (0 hours) or after 24, 48 or 72 hours. Data are the mean ± s.d. from three or nine mice of each genotype. (B) Northern blot analysis of Hrk gene expression on polyA+ mRNA extracted from IL3-dependent haematopoietic cell lines and their derivatives over-expressing Bcl2. Each cell line was deprived of IL3 for the indicated times. PolyA+ mRNA from neonatal brain was used as a positive control. Northern blotting for the gene encoding Bim was included as a control to show that each cell line was stimulated to upregulate BH3-only gene expression. Gapdh RNA was loaded as control. (C) Northern blot analysis of Hrk gene expression on polyA+ mRNA extracted from the indicated tissues of C57BL/6 mice. Gapdh RNA was detected as a loading control.





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