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Figure 5


Fig. 5. Loss of HRK does not protect cerebellar granule neurons from K+ deprivation. (A) Cerebellar granule neurons were isolated from wild-type (Hrk+/+, n=3) and Hrk–/– (n=3) littermates, cultivated in high-K+ medium for 7 days, then transferred to low-K+ medium to induce apoptosis. Viability was determined 0, 6, 12 and 24 hours after transfer to low K+ using the Live/dead assay (Molecular Probes) to assess the number of viable and dead neurons in five independent fields at each time point for each animal and expressed in percent. Data are the mean ± s.d. (B) Q-PCR analysis of Hrk mRNA expression in wt cerebellar granule neurons cultured for 4 hours in the presence of high (K25+S) or low levels (K5+S) of K+. Expression of neurofilament 3 was used for normalisation. Data are the mean ± s.d. from nine cultures of neurons from three mice (triplicates).





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