CTGF enhances the motility of breast cancer cells via an integrin-v3–ERK1/2-dependent S100A4-upregulated pathway

JCS03460 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1A
• Supplemental Figure S1B
• Supplemental Figure S1C -

  Fig. S1. CTGF regulates integrin α_vβ₃ in human breast cancer cells. (A) Integrin-subunit expression levels in T47D, MCF-7- and MDA-MB-231-derived cell lines. Expression of integrin subunits were detected in MCF-7/neo, MCF-7/CTGF, MDA231/neo, MDA231/AS, and T47D cells by RT-PCR. (B) Distribution of integrin α_vβ₃ in MCF-7- and in MDA-MB-231-derived cell lines. Serum-starved MCF-7/neo, MCF-7/CTGF, MDA231/neo, and MDA231/AS cells were fixed in 3.7% paraformaldehyde. Fixed cells were co-stained using anti-integrin α_vβ₃ antibody (left panel) and DAPI (right panel). (C) Integrin α_vβ₃ expression levels in T47D, MCF-7- and MDA-MB-231-derived cell lines. Expression of integrin subunits was detected in MCF-7/neo, MCF-7/CTGF, MDA231/neo, MDA231/AS, and T47D cells by flow cytometry. Flow cytometry analysis was carried out with anti-integrin α_vβ₃ or its isotype antibodies to examine cell-surface expression of the integrin α_vβ₃ in these cells.

• Supplemental Figure S2 -

  Fig. S2. Expression of E-cadherin in MCF-7- and in MDA-MB-231-derived cell lines. Level of E-cadherin was detected in MCF-7/neo, MCF-7/CTGF, MDA231/neo, and MDA231/AS cells by western blotting.

• Supplemental Figure S3 -

  Fig. S3. Expression of S100A4 in human breast cancer cells. Levels of S100A4 were detected in BT474, BT483, T47D, MDA-MB-453, MCF-7, MDA-MB-231, and MDA-MB-435 cells by northern blotting.