spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. CTGF-mediated outside-in signaling confers enhanced migratory ability in human breast cancer cells. (A) Effect of rCTGF on the migratory ability of MCF-7 cells. The migratory ability was measured by using a Boyden chamber assay with rCTGF or without (solvent control). Columns show the mean of three independent experiments, and error bars are the corresponding upper 95% confidence intervals. Asterisks denote a statistically significant difference in the migratory ability of treated cells compared with that of control cells (*P<0.05, **P<0.01, two-tailed Student's t test). (B) Conditioned medium (C.M.) was collected from MDA-MB-231 cells 48 hours after plating. The migratory ability of MCF-7 cells was measured by Boyden chamber assay with CTGF-neutralizing antibody or without (normal rabbit IgG) of a CTGF-neutralizing antibody. Columns show the mean of three independent experiments, and the error bars are the corresponding upper 95% confidence intervals. Asterisks denote a statistically significant difference in migratory ability of treated cells as compared to control cells (*P<0.05, **P<0.01, two-tailed Student's t-test, a, differences in migratory ability between treated cells and untreated cells, b, differences in migratory ability between CTGF-neutralized antibody-treated cells and normal rabbit IgG-treated cells). (C) Blockade of CTGF outside-in signaling inhibits the migratory ability of MDA-MB-231. The migratory ability of MDA-MB-231 cells was measured by using a Boyden chamber assay with CTGF-neutralizing antibody or without (normal rabbit IgG). Columns show the mean of three independent experiments, error bars are the corresponding upper 95% confidence intervals. Asterisks denote a statistically significant difference in migratory ability of treated cells as compared to control cells (*P<0.05, ** P<0.01, two-tailed Student's t-test). (D) Blockade of CTGF outside-in signaling result in morphological alterations and cytoskeleton rearrangements. Serum-starved MDA-MB-231 cells were untreated or treated with CTGF-neutralized antibody or isotype control (normal rabbit IgG). Cells were fixed in 3.7% paraformaldehyde and photographed under a light microscopy (a,e,i). The fixed cells were co-stained with Texas-Red-phalloidin for F-actin (red; b,f,j) and monoclonal antibody against paxillin (green; c,g,k); overlays are shown in d,h,c. (E) CTGF expression resulted in enhanced migratory ability through integrin {alpha}vβ3. Cells were pretreated with integrin {alpha}vβ3-blocking antibody or without (normal rabbit IgG) or for 6 hours prior the treatment with rCTGF. The migratory ability of MCF-7 cells was measured by the Boyden chamber assay. Columns show the mean of three independent experiments, error bars are the corresponding upper 95% confidence intervals. Asterisks denote statistically significant difference in migratory ability of treated-cells compared with that of control cells (*P<0.05, ** P<0.01, two-tailed Student's t-test, a, differences in migratory ability between rCTGF-treated cells and untreated cells, b, differences in migratory ability between CTGF-neutralized antibody-treated cells and IgG-treated cells).





Right arrow Return to article