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Fig. 1. Molecular genetic analysis of the mlp84B locus. (A) Identification of deficiencies that remove mlp84B. DNA from several deficiency lines in the 84B,C region was digested with EcoRI, and probed with the mlp84B cDNA. The second blot contains DNA from deficiency lines that harbor a balancer chromosome with a restriction fragment length polymorphism in the mlp84B region (TM3^*). Loss of the larger EcoRI band (10 kb) indicates a deletion of mlp84B. (B) Southern blot analysis of the mlp84B^P8 allele showing deletion of the mlp84B transcription unit. The arrangement of the HindIII fragments relative to the mlp84B transcription unit is shown to the right [arrow indicates band contributed by the Df(3R)dsx2M chromosome]. (C) Molecular map of the 84C1,2 region. Deficiencies used to generate mlp84B-null animals are shown above the map, and the deletion in mlp84B^P8 is also shown. The mlp84B transcription unit is shown in detail below the map, with the position of the P-element in mlp84B^P20 indicated. (D) Western blot of protein lysates from third-instar larvae demonstrating loss of the mlp84B gene product in mlp84B mutants. 10 µg of protein was loaded per lane. Genotypes for the larvae are indicated on the right; 4B2 is the mlp84B transgene, and 2X4B2 indicates two copies of the transgene. The open arrow indicates a weak non-specific band detected by this antiserum.

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