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Fig. 2. Loss of Mlp84B affects muscle-driven morphogenetic processes. (A) Pupal lethality associated with loss of mlp84B and rescue with the mlp84B transgene. (B) Axial ratios for pupae of indicated genotype. [*P>10–5, compared with two-copy rescue line (4B2 Scx2/4B2 dsx2M).] Data are displayed as a distribution of data points for each pupae, bounded by the upper quartile and lower quartile, with the median value indicated as a line in the box. Error bars show the upper and lower limits of the data, and any outliers are indicated as a circle. (C) Brightfield images of wild-type and Df(3R)dsx2M/Df(3R)Scx2 pupae (mlp84B–/–); bar, 200 µm. Anterior is to left. Arrow indicates air pocket that failed to translocate. Arrowhead shows failure in spiracle eversion. (D) Scanning electron microscopy image of wild-type and Df(3R)dsx2M/Df(3R)Scx2 pupae; bar, 200 µm. Arrowhead shows failure in spiracle eversion.