JCS004127 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Nodal-expressing ES cells induce TGFβ, activin (A) and BMP (B) ligands.

• Supplemental Figure S2 -

  Fig. S2. Nodal-expressing ES cells at stage 2 increase the expression of the gene products for the endoderm and mesoderm lineage markers. Immunofluorescence microscopy images showing Foxa2, CXCR4 and VEGFR2. The original magnification was 400×.

• Supplemental Figure S3 -

  Fig. S3. Immunofluorescence images of the mesoderm markers, Flk-1, VE-cadherin, and α-smooth muscle actin (αSMA) in Nodal on/off grafts. The original magnification was 400×.