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Figure 4


Fig. 4. Sustained Nodal expression inhibited the maturation of definitive endoderm. (A) RT-PCR showing expression of the pluripotent marker, Oct4, at stages 2, 3a and 3b. (B) Immunohistochemistry showing Oct4 and SSEA1 expression at stage 3. (C) Quantitative single cell analysis by FACS for definitive endoderm and mesoderm progenitor cells at stage 3. The CXCR4+GFP+, VEGFR2+GFP+ and PDGFR{alpha}+GFP+ cell populations decreased in stage 3a ES cells. By contrast, the CXCR4+GFP+ cell population in stage 3b ES cells was unchanged in comparison to that of stage 2 Nodal upregulated ES cells.





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