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Figure 3


Fig. 3. Nucleolin depletion induces spindle-checkpoint activation. (A) Mitotic indexes of control cells and cells depleted of nucleolin by siRNA1 or siRNA2. Values represent the mean of three independent experiments ± s.d. (n=400). (B) Mitotic indexes of control, nucleolin-depleted, Aurora-B-depleted and both nucleolin- and Aurora-B-depleted cells at 72 hours after control and RNAi transfections. Values represent the means of three independent experiments ± s.d. (n=400). (C) Percentages of control and nucleolin-depleted cells at different stages in the cell cycle. Values represent the mean of four independent experiments ± s.d. (n=400). (D) Mitotic cells stained for Bub1 (green) and {alpha}-tubulin (red). (E) Mitotic cells stained for BubR1 (green) and {alpha}-tubulin (red). (F) Mitotic cells stained for Mad2 (green) and {alpha}-tubulin (red). Arrowheads in D,E and F show misaligned chromosomes and the fluorescence signals for Bub1, BubR1 and Mad2 at the kinetochores, respectively. Bars, 5 µm.





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