spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Nucleolin depletion induces significant reductions in centromere stretching and syntelic kinetochore-microtubule attachments. (A) Analysis of the interkinetochore distances across sister chromatids. Immunostaining for Aurora-B (central) and CENP-A (either side of Aurora-B) is shown in red and green, respectively. Bars, 5 µm; 1.25 µm (enlarged area). (B) Interkinetochore distances of non-aligned and aligned chromosomes following siRNA transfections as indicated. The interkinetochore distances were measured using CENP-A as a marker. Each value was derived by measuring at least 40 kinetochores in more than five cells. Values for non-aligned chromosomes in control cells were obtained at prometaphase. (C) Control metaphase cells stained with CREST (red) and {alpha}-tubulin (green) antibodies. (D,E) Misalignment (D) and non-alignment (E) phenotypes stained with CREST (red) and {alpha}-tubulin (green) antibodies. (F) Cells were cold-treated and stained with {alpha}-tubulin (green) and CREST (red) antibodies.





Right arrow Return to article