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Figure 6


Fig. 6. Nucleolin depletion induces a mitotic block at prometaphase. (A,B) HeLa cells stably expressing histone GFP-H1.2 were imaged by time-lapse fluorescence microscopy after transfection of the indicated siRNAs. Times are given in minutes. DIC, differential interference contrast. (C) Histogram showing the percentages of cells with different durations of mitosis. Fifty-three control cells and 58 nucleolin-depleted cells were analyzed for time-lapse fluorescence microscopy. (D) HeLa cells stably expressing GFP-CENP-A were imaged by time-lapse fluorescence microscopy after transfection of the indicated siRNAs. Times are given in minutes and seconds. Bars, 10 µm.





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