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Figure 8


Fig. 8. Immunoblot analysis of nucleolin-binding complexes from total cell extracts and synchronized mitotic cell extracts. Nucleolin-binding complexes were pulled down from total cell extracts (A) and synchronized mitotic cell extracts (B) by an anti-nucleolin antibody. To obtain the synchronized mitotic cell extracts, cells under double-thymidine block were released for approximately 10 hours, and then lysed for immunoblotting detection. Protein G beads coupled with mouse IgG were used as a control.





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