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Figure 7


Fig. 7. Nek2 controls centrosomal NIP2 levels. (A) HeLa cells were transfected with siCTL, siNIP2 or siNek2. Twenty-four hours after transfection, cells were co-immunostained with antibodies against NIP2 and Nek2. Insets are magnified views of the centrosomes. (B) HeLa cells were transfected with siCTL or siNek2, and their cell cycles were arrested at the G1/S boundary with a double-thymidine block. The cells were then immunostained with NIP2 antibody to determine centrosomal NIP2 levels. More than 300 cells were analyzed per group in three independent experiments. FACS histograms show synchronization of HeLa cells at the G1/S boundary. (C) The amount of cellular Nek2 increased due to overexpression of HA-tagged wild-type Nek2 (Nek2RHA1) or decreased due to transfection of siNek2. Twenty-four hours after transfection, cells were immunostained with antibodies against NIP2 and HA. The amount of the centrosomal NIP2 was determined by fluorescent intensity. More than 300 cells were counted in three independent experiments.





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