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First published online June 5, 2007

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Nucleolin on the edge

Between prometaphase and telophase, each chromosome is surrounded by a layer of proteins and RNA. This mysterious `chromosome periphery' is thought to be involved in protein transport and maintenance of chromosome structure, but its roles are not well defined. On p. 2091, Kiichi Fukui and colleagues shed light on the function of one protein associated with the chromosome periphery - nucleolin. Nucleolin is an important component of the nucleolus, and its roles in ribosome production and assembly have been studied extensively. To investigate its function during mitosis, the researchers used antibodies to track its dynamics through the cell cycle. During interphase, nucleolin is associated with nucleoli, but between prometaphase and telophase it relocalises to the chromosome periphery - in particular near the outer kinetochores (the complexes that attach chromosomes to spindle microtubules) and the spindle poles. Using RNAi together with live- and fixed-cell imaging, the researchers found that cells lacking nucleolin have disorganised nucleoli and a prolonged cell cycle, and their chromosomes fail to congress. They show that the delay is due to a defect in kinetochore-microtubule interaction, concluding that nucleolin has roles not only in nucleolus formation but also in chromosome congression and spindle formation.

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Related articles in JCS:

Nucleolin functions in nucleolus formation and chromosome congression

Nan Ma, Sachihiro Matsunaga, Hideaki Takata, Rika Ono-Maniwa, Susumu Uchiyama, and Kiichi Fukui
JCS 2007 120: 2091-2105. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?