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Figure 6


Fig. 6. cav1-MO disrupts development of neuromasts in the posterior lateral line. (A) Projected stack of confocal sections of zebrafish embryos labelled with caveolin antibody shows labelling around the neuromasts near the eye (e) at 72 hours. Cav1 whole-mount in situ hybridisation was carried out and sections of 72-hour embryos were examined. (B) cav1 mRNA is found throughout the neuromast. (C) Scanning electron micrograph of a 72-hour control zebrafish embryo with normal neuromasts. DASPEI labelling (D-F) and scanning electron microscopy (G-J) reveal that 72-hour control MO injected embryos have seven to eight neuromasts along their posterior lateral line (D) but when they are injected with a morpholino targeted to cav1{alpha} at 1.5 ng/embryo (cav1-MO1), the number of neuromasts are severely reduced (E). (F) The result was confirmed with the second morpholino to cav1{alpha} (cav1-MO2). (G-J) The number of neuromasts in the posterior lateral line after cav1 MO injection was also shown to be reduced (H,J) compared with WT embryos (G,I). I and J are magnifications of the boxed areas of G and H to demonstrate the loss of neuromasts in the lateral line. h, head. Bars, 20 µm (A-C); 250 µm (D-H); 100 µm (I,J).





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