spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Reduced production of bone extracellular matrix and decreased osteoblast proliferation in Smad4 mutant mice. (A-D) In situ hybridization of proximal tibias from 4-week-old control (A,C) or mutant mice (B,D). The expression of (B) osteopontin (OPN) and (D) osteocalcin (OC) was decreased in Smad4 mutant mice compared with controls (A,C). (E-H) Real-time PCR of Akp1 (ALP), osteocalcin, Col1a2 and Runx2 from calvarial bone extracts of 7-week-old Smad4 mutant (grey bars) and control mice (white bars). Values are presented as relative expression. (I) Sections of calvaria from 3-week-old mice showing a reduced number of BrdU-positive cells (arrowhead) in Smad4 mutant mice compared with control littermates. (J) Osteoblast mitotic index (percentage of BrdU-positive cells per total cell number) in control (white bars) and Smad4 mutant mice (grey bars). *P<0.01. Bar, 400 µm (A-D); 26.5 µm (I).





Right arrow Return to article