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Figure 1


Fig. 1. Expression of sGi2 reduces the dopamine D2 receptor density at the plasma membrane. (A) Infection of BHK cells with 30 µl pseudovirions of D2L (long isoform of D2 receptor) and 0-15 µl of sGi2 (bullet) or G{alpha}i2 (Gi2, {blacksquare}) protein showed a gradual decrease in the number of plasma-membrane-bound D2 receptors as the expression of sGi2 protein was increased. Gi2 had no effect. (B) Immunoblots of cells from A show that, as the addition of sGi2 pseudovirion was increased, higher expression of this protein. D2L expression remained the same. Non-transfected cells are shown in lane 4. (C) In JEG-3 cells that lack endogenous Gi2 protein, co-expression of sGi2 with either D2L or D2S (short isoform of D2 receptor) receptors also yielded a 32-35% reduction in cell surface D2 receptors, similar to that as seen in BHK cells in A. *P<0.05, significant change from control. Values are representative of six to eight different experiments.





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