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Figure 6


Fig. 6. The extreme C-terminal end of sGi2 is essential for the intracellular interaction. (A) For deletion experiments, sGi2 constructs were prepared by eliminating the part of the complete cDNA sequence with the use of specific PCR primers and then cloned. Each bar reflects the approximate size and location of each construct included in the study. Numbers on the right indicate start and end of the cDNA sequence. sGi2 (WT) and G{alpha}i2 (WT) are complete genes and rest (c-f) are the constructs derived from sGi2 (WT). Shaded areas show the sequences that differ between sGi2 and G{alpha}i2. G{alpha}i2 was included in the study as control. (B) Each of the constructs or WT (a-f) from (A) was co-expressed with D2S receptor in BHK cells and the receptor density at cell surface was assayed in intact cells. The protein expression of these constructs was confirmed by western blots (see supplementary material Fig. S5).The 108 base pairs on the extreme 3'-end were necessary for the intracellular interaction and retention of the receptor. Values are representative of five different experiments.





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