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Figure 1


Fig. 1. (A) The titin genomic region spans from the 5' end of the predicted zormin ORF through the 3' end of the predicted sallimus/titin ORF. Both of the ORFs predicted from zormin and sallimus/titin have significant homology to vertebrate titin and to the multiple C. elegans titin-like proteins. (B) The domain structure of the predicted composite titin protein, which would include 21139 residues, contains 55 immunoglobulin repeats (Ig; blue), five fibronectin type-3 repeats (FN3; silver) and two extended domains rich in Pro, Glu, Val and Lys (PEVK-1 and PEVK-2; yellow). Polyclonal antiserum was generated to three non-overlapping fragments from the composite protein (52, KZ and 56; green). The colored line above the domain structure indicates which regions of the protein are encoded by the three Celera Genomics Predicted genes: zormin (yellow), CG32307 (purple) and sallimus/titin (red). (C) Drosophila embryos stained with antisera generated to protein fragments corresponding to the zormin coding region (anti-52) and to the N-terminal region of the sallimus/titin coding region (anti-KZ) show identical patterns of accumulation, with staining in the skeletal, pharyngeal and visceral muscle, as well as in the muscle attachment points, known as apodemes (left and middle panels). Drosophila embryos stained with antiserum raised against a more central fragment of the sallimus/titin coding region show the same pattern of accumulation except that staining is not detected to high levels in the visceral muscle (right panels).





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