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Figure 11


Fig. 11. Megator and chromator distribution in crane-fly and locust spermatocytes. (A,D,G,J) Prophase spermatocytes illustrating megator (A,G) and chromator (D,J) staining of chromosomes. (B,E,H,K) Metaphase spermatocytes double stained for megator and tubulin (B,H) and for chromator and tubulin (E,K), illustrating spindle fiber staining. Left panels in B,E,H,K show megator or chromator staining, middle panels show tubulin staining and right panels show the merged image of the two channels (green, megator or chromator; red, tubulin; yellow-orange, co-localization). Inserts in E are enlarged images of the boxed regions and illustrate differences between chromator and tubulin: chromator has a fine granular appearance along spindle fibers, whereas tubulin has a filamentous appearance. (C,F,I,L) Telophase/cytokinesis spermatocytes illustrating megator (C,I) and chromator (F,L) staining of midbody, newly formed nuclei and contractile ring (L). Bars, 5 µm.





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