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Figure 12


Fig. 12. A prometaphase crane-fly spermatocyte stained for D-titin with anti-KZ antibody (green) and for skeletor (red). (A) D-titin and skeletor co-localize (yellow-orange) along kinetochore and non-kinetochore spindle fibers and at the poles (asterisks). Arrow indicates one autosomal bivalent. (B) Enlarged details of the boxed region in A showing D-titin vs skeletor distribution along spindle fibers in five consecutives slices to illustrate their spatial localization in the Z-axis. Arrows indicate one sex chromosome. Open arrowheads indicate D-titin vs skeletor localization along non-kinetochore microtubules. Bar, 5 µm.





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