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Figure 2


Fig. 2. Distribution of anti-D-titin antibodies in glycerinated myofibrils from crane-fly flight muscle. (A) An example of the method we used to identify Z-lines in confocal images. The same myofiber is illustrated in a phase-contrast image, a DIC image and a confocal microscope image. The brackets show the same sarcomere in the three images and the arrowheads point to Z-lines, which delimitate the sarcomere. (B-D) In general, the Z-lines (arrows), identified from corresponding DIC and phase-contrast images, are brightly stained. There is diffuse staining in between, along the length of the sarcomere. The staining patterns of all three antibodies vary with the sarcomere length, as described in text. Bars, 5 µm.





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