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Fig. 9. Distribution of anti-KZ antibody (green) vs actin distribution (red) in insect spermatocytes. (A-C) Merged channels of D-titin and actin staining in prometaphase (A), metaphase (B) and anaphase (C) crane-fly spermatocytes (Z-series), showing their co-localization (yellow-orange) in the chromosomes (arrows), at the poles (arrowheads) and along the spindle fibers. (D,E) Merged channels of D-titin and actin staining in prophase (A) and metaphase (B) locust spermatocytes (Z-series), showing their co-localization (yellow-orange) in the chromosomes (arrow) and along the spindle fibers. Bars, 5 µm.