spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Activation of new growth zones in G1- and S phase-arrested cells. (A) Images of cdc10-129 cdc11-119 cells at 36.5°C, after treatment with 50 µg/ml MBC for 3 hours: Nomarski (top) and DAPI stained (bottom). (B) Scoring of branching cdc10-129 and cdc10-129 cdc11-119 cells. Both cdc10-129 and cdc10-129 cdc11-119 cells were blocked for 3 hours before MBC treatment. Black arrow indicates drug addition. (C) Scoring of branching cdc25-22 cells. Cells were blocked for 3 hours at the restrictive temperature to block cells in G2 prior to mitosis. After 3 hours cells were released to the permissive temperature (25°C) to allow mitosis in the presence of 12 mM HU to prevent S phase to proceed. After 1.5 hours DMSO/MBC was added to the media. The effect of temperature and HU treatment was controlled using a wild-type strain. Both treatments had no effect on wild-type cells (data not shown). (D) Scoring of monopolar and bipolar cells in cdc25-22 cells blocked at 36.5°C and then released to 25°C in 12 mM HU. Bars, 5 µm.





Right arrow Return to article