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Figure 1


Fig. 1. Effects of hypoxia on Na,K-ATPase endocytosis in A549 cells. (A) Serum-starved A549 cells were exposed to 21 or 1.5% O2 for 60 minutes and the protein levels of the Na,K-ATPase-{alpha}1 subunit or the transferrin receptor in the plasma membrane were studied by cell surface biotinylation followed by streptavidin pull-down and western blot analysis using specific antibodies. A representative western blot for each protein is shown, mean ± s.e.m. (n=4). **P<0.01. (B) COS-7 cells were transiently transfected with vector or GFF-Dyn K44A, serum-starved and exposed to 21 or 1.5% O2 for 60 minutes. Na,K-ATPase endocytosis was studied by biotin labeling of surface proteins followed by streptavidin pull-down and western blot analysis using a specific anti {alpha}1-subunit antibody. Top: bars represent mean ± s.e.m. (n=3), **P<0.01. Bottom: a representative western blot for {alpha}1-Na,K-ATPase endocytosis and GFP-dynamin expression levels.





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