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Fig. 1. (A) A typical force vs extension curve. The dotted line corresponds to the approach curve and the solid line is the retraction curve. On the retraction curve, several step-like structures are clearly discernible, which correspond to the sequential detachment of individual tethers from the cantilever. (In principle these steps could signal the detachment of a single tether with multiple connections to the cantilever. This possibility can be excluded on the basis of the numerous earlier studies in which single tethers were extracted and no steps in the course of tether elongation were observed.) The shown retraction curve corresponds to an experiment performed with a control cell at 3 µm/second. (B) Histogram constructed from the data (size of the steps) collected in the control experiments at 3 µm/second. The solid line is the Gaussian fit to the histogram, which gives the mean value of the tether force, 33 pN.
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