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Figure 2


Fig. 2. Recombinant AtMAP70-1 and AtMAP70-5 co-sediment with pre-assembled microtubules in vitro. Lanes 1 and 6: extracts of E. coli cells expressing 6xHis-AtMAP70-5 and 6xHis-AtMAP70-1, respectively; concentrations of the purified proteins were estimated as 50 µg/ml for 6xHis:AtMAP70-1 and 10 µg/ml for 6xHis:AtMAP70-5 using SDS-PAGE and BSA standards (not shown). Lane 7: purified tubulin used to form microtubules, but before assembly with Taxol. Lanes 2-5: co-sedimentation of 6xHis-AtMAP70-5 with pre-assembled microtubules. Lanes 8-11: co-sedimentation of 6xHis-AtMAP70-1 with pre-assembled microtubules; P, pellets (lanes 2, 4, 8 and 10); S, supernatants (lanes 3, 5, 9 and 11); + Mts, pre-assembled microtubules added to the reaction mixture (lanes 2, 3, 8 and 9); – Mts, no pre-assembled microtubules added (lanes 4, 5, 10 and 11).





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