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Figure 2


Fig. 2. DRM-tagged CLIMP-63 related constructs expressed in M3/18 cells have the molecular mass expected. (A) Schematic representation of CLIMP-63 related constructs tagged with the red fluorescent protein (DRM) of Discosoma sp. CLIMP-63 is a type II transmembrane protein. It has a relatively short N-terminal cytosolic domain (hatched box), which is capable of binding to microtubules. A larger C-terminal lumenal domain (open box) can form oligomers. The transmembrane domain is represented by a black box. Monomeric DRM used as a tag is represented as a gray oval. (B) The cells transfected with recombinant plasmids encoding p63-DRM (lane 1), {Delta}Lp63-DRM (lane 2), DRM-{Delta}Cp63 (lane 3), {Delta}TLp63-DRM (lane 4) or the cloning vector encoding DRM (lane 5) were subjected to western blot analysis using a commercial M3/18 directed against the red fluorescent protein. All expressed fusion proteins had the expected molecular mass.





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