spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Impaired tight junction closure at secretory activation in PKN1 transgenic mice. (A) Increased permeability of mammary tight junctions in PKN1 transgenic mice. [14C]sucrose was injected into the milk duct of animals of the indicated genotype at the first postpartum day, blood samples were taken 5 minutes after the injection and counted for 14C. The displayed results depict averages and s.e.m. from four wild-type and five transgenic animals (*P<0.05, Student's t-test). (B) Immunohistochemical detection of the tight-junction-associated molecule ZO-1 in wild-type and transgenic mice on the first day of lactation. ZO-1 was visualized with a secondary antibody coupled to Cy3; nuclei were stained with DAPI. To avoid focus artifacts, z-stacks were taken and projected into a two-dimensional image. Broadened ZO-1 staining is marked with an arrow.





Right arrow Return to article