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Figure 4


Fig. 4. Effect of deficiency in p53 on intracellular ROS level in the SiHa cell line. (A) Intracellular ROS levels in SiHa cells measured by CM-H2DCFDA staining and FACS analysis at different time intervals after transfection with p53 siRNA or nonspecific siRNA. (B) ROS levels expressed as the mean ± s.d. intensity of cell fluorescence. *P<0.001 compared with the corresponding negative control by Student's t-test. (C) FACS analysis of CM-H2DCFCA-stained cells treated with NAC (5 mM, 6 hours) 48 hours after transfection with p53 siRNA or nonspecific siRNA. *P<0.01 compared with the corresponding untreated group by Student's t-test. (D) Cytosolic superoxide levels in SiHa cells transfected with p53 siRNA or nonspecific siRNA measured by DHE staining and FACS analysis. (E) Mitochondrial superoxide levels in SiHa cells transfected with p53 siRNA or nonspecific siRNA measured by MitoSOX RED staining and FACS-analysis.





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