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Fig. 6. ROS levels in HeLa (A), CaSki (B) and ME180 (C) cells 48 hours after inhibition of p53 with siRNA (sip53). For a negative control we used nonspecific siRNA. *P>0.95 compared with the corresponding cells with negative control vector (Student's t-test). (D) Expression levels of p53 mRNA in both control and si-p53-expressing cells 48 hours after transfection with corresponding plasmid, as detected by RT-PCR.